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The Pathological Mechanisms and Treatments of Tinnitus

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About this article Cite this article Main, P.A., Angley, M.T., O'Doherty, C.E

The peptide chains remain intact

the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C